blastocysts

After the blastocysts were hatched naturally from the zone pellucida, the inner cell mass ( ICM) was dislodged from blastocysts using the sealed end of a finely drawn Pasteur pipette and co cultured with the feeder cells.

当囊胚由透明带自然孵出后，用细玻璃针剥离囊胚中的内细胞团（inner cell mass，ICM）并与饲养细胞进行共培养。

Results: There was high rate of attachment of blastocysts that were indigested external mucoprotein and partial zona pellucida in 96 h culture ( 85.29%, 29/ 34) and growth of ICM was excellent;

结果显示：消化掉外层粘蛋白和部分透明带的胚胎96h贴壁率（85.29%，29/34）较高，内细胞团生长良好；

The introduction of thecal cells either in suspension or in the form of the monolayer or conditioned medium to the maturation system resulted in an increase in the proportion of oocytes developing to blastocysts ( 44.1%, 42.6% and 39.8% resp.).

加入5×10~6/mL内膜细胞、内膜细胞的单层细胞或25%条件液到成熟培养系统中，卵母细胞的囊胚发育率均有所提高，分别为44.1%，42.6%及39.8%。