unclamped

Methods: All rats were subjected to openning chest then the left anterior desending coronary arteries ( LAD) were ligated for 30 min and unclamped for 60 min, measure the apoptotic cells by TUNEL, and detect the Caspase-3 by immunohistochemistry.

方法：采用在体大鼠结扎冠状动脉前降支30min后，松扎再灌注60min造成心肌缺血再灌注模型，使用TUNEL法进行细胞凋亡检测，使用免疫组化法测定凋亡相关蛋白Caspase3水平。

To induce intestinal venous congestion reperfusion model, portal vein was clamped for 45 min, then unclamped for 60 min. This reperfusion model was made in CR group and in Lipo-PGE1 group.

CR组和Lipo-PGE1治疗组均制作肠道急性淤血再灌注模型。模型制作：先阻断门静脉45min，再恢复血流60min。

The left anterior descending coronary arteries were ligated and then unclamped to establish ischemia-reperfusion injury model. The ischemic preconditioning was induced by three cycles of 5 minute ischemia/ 5 minute reperfusion.

阻断和松开左冠脉前降支制作缺血再灌注模型，缺血5min/再灌注5min连续3次循环诱导预适应。