thiolase

Designed the specific primers to amplify by PCR, sequencing and splicing, acquired genome DNA sequences coded 3-ketoacyl-CoA thiolase of the sch silkworm.

设计特异引物进行常规PCR扩增，通过测序、拼接，获得了sch蚕编码酮酯酰CoA硫解酶的完整DNA序列。

Result showed that there are 15 nucleoside acid differences with normal silkworm DNA sequence of 3-ketoacyl-CoA thiolase; mere are 5 amino acid differences after translated.

结果是与正常家蚕编码酮酯酰CoA硫解酶的DHA序列有15个核苷酸的差异，翻译后有5个氨基酸的差异。

Those suggest that electrostatic screen, hydrophobic effect and surface tension all contribute to halophilic properties of thiolase.

这表明极端嗜盐硫解酶的嗜盐特性不单来自形成的分子静电屏蔽网和疏水作用的调节，且与分子表面张力减小密切相关。