dilutions

The method is: series dilutions of a plasmid with known concentration were used to establish the standard curve and to measure the amplification efficiency of the system.

具体方法是采用浓度已知的DNA作为标准品建立标准曲线，与未知样本在同一反应板上同时定量，标准曲线为6个点。在建标准曲线同时，确定实时PCR的扩增效率；

The standard curves were made by real-time quantitative PCR reaction using the dilutions of RNA as the template.

将RNA标准品梯度稀释作为模板进行实时荧光定量PCR反应，制作标准曲线。

The MIC of nisin to Streptococcus thermophilus was determined by serial twofold dilutions in skim milk medium and whey medium.

采用两倍稀释法在脱脂乳培养基和乳清培养基中测定了nisin对嗜热链球菌9的最小抑菌浓度（MIC）。