I-site的翻译

Second, base on the foundation of questionnaire and on-site inspection, I give a present condition description of each county City of the South Fukien overseas Chinese's home town, and announced some problem that exhibition exist nowadays.

其次在问卷调查与实地调查的基础上，对闽南侨乡各县市的城镇居民进行社区体育健身锻炼的开展进行了现状描述，并揭示了目前闽南侨乡社区体育开展所存在的一些问题。

Restriction enzyme assay, PCR and sequencing confirmed that the TK gene was obtained successfully. Only one Kpn ⅰ site and a transcription termination signal TTTTT N ( T) T were found in the gene.

酶切鉴定、PCR鉴定和测序结果表明，成功获得了TK基因，获得的TK基因内存在一个KpnⅠ酶切位点和编码区的早期转录终止信号TTTTTN（T）T。

The Kpn ⅰ restriction site was introduced into the both ends and a random linking peptide of 3 amino acids into 3 ′-end.

并在突变体片段两端引入KpnⅠ位点，3′端引入3个氨基酸大小随机连接肽的序列。