transcriptase

After sacrificing the rats in twelve weeks, serum and aorta were studied through the techniques: biochemistry, radioimmunoassay, immuno-histochemistry ( IHC), reverse transcriptase PCR ( RT-PCR) and western blot.

各组大鼠实验12周后处死，提取血清、主动脉、通过生化、放免、免疫组化、RT&PCR法及蛋白印迹杂交法进行研究。

Methods Total RNA in the metastatic lymph nodes from patients with colorectal cancer was extracted routinely, and reverse transcriptase PCR employed to amplify the heavy chain Fd and light chain κ cDNA.

方法取大肠癌病人转移淋巴结，提取淋巴结总RNA，逆转录PCR扩增重链Fd和K轻链cDNA。

Extraction of total RNA from culture clones which were expanded, and then detecting the target gene was integrated into the genome of HeLa cells through reverse transcriptase PCR method ( RT-PCR).

对单克隆进行扩大培养，提取其总RNA，逆转录酶PCR法（Reverse transcriptase-PCR，RT-PCR）检测到目的基因已整合到HeLa细胞基因组中，说明重组质粒在细胞中稳定表达。