parthenogenetic

in this study the effects of concentration of srcl 2, duration time of exposure to srcl 2 and oocyte age on the parthenogenetic activation of mouse oocytes were studied.

以昆明小鼠为试验对象，研究了氯化锶（SrCl2）浓度、激活处理时间以及卵龄对卵母细胞SrCl2孤雌活化的影响。

These results indicate that CHX at an appropriate concentration can improve the parthenogenetic development of buffalo oocytes, CB at an appropriate concentration may inhibit the extrusion of the first polar body and increase the proportion of activated oocytes with two pronuclei. 2.

结果表明，适当浓度的CHX能提高卵母细胞激活效果，适当浓度的CB可抑制卵母细胞第二极体的排出，提高孤雌激活后双原核的比例。

Ionomycin with different concentrations was used for different time; Ionomycin was combined with 6-DMAP, CB and CHX respectively; 9% Ethanol was used for different time. The efficiency of each combination was compared to study the better chemical parthenogenetic activation method.

不同浓度Ionomycin作用不同时间；Ionomycin分别与6-DMAP、CB及CHX组合；9%Ethanol作用不同时间，对每一组合的激活效率进行比较，旨在研究较好的化学激活方法。