mutational助记

the mutational biosynthesis of doramectin and studies on the fermentation condition

多拉菌素的突变生物合成及发酵条件优化

methods: based on the base sequence of the g-protein polypeptide, the oligonucleotide primers were designed and synthesized which contains the mutational nucleotide sequence.

方法本文根据G蛋白多肽碱基序列的需要，设计并合成了带有突变核苷酸序列的寡聚核苷酸引物，以G基因RT-PCR产物为模板，采用高保真DNA聚合酶，PCR突变扩增，得到G蛋白多肽编码序列。

Roles of the EnvZ/ OmpR two-component system in response to various stresses were investigated with mutational analysis, quantitative reverse transcriptase PCR ( qRT-PCR).

同时通过突变分析、定量逆转录聚合酶链反应（qRT-PCR）研究了EnvZ/OmpR二元系统在应对渗透压力中的作用。