house-keeping

[ METHOD] In this paper, three gene S-adenosylmethionine decarboxylase ( SAMDC), ornithine aminotransferase ( OAT) and betaine aldehyde dehydrogenase ( BADH) plus one house-keeping gene had been cloned and their expression levels under different stresses were evaluated by real-time PCR technique.

【方法】已克隆S-腺苷蛋氨酸脱羧酶、鸟氨酸氨基转移酶、甜菜碱脱氢酶3个基因和内参基因的基础上，应用定量PCR技术，对这3个基因在不同胁迫时间下的表达水平进行分析。

Immunohistochemical staining for cyclin D1 and semi-nested PCR for t ( 11; 14) were detected in all samples. House-keeping gene β-actin was used to detect the quality of DNA.

用半巢式聚合酶链反应（PCR）法检测t（11；14）易位，以看家基因β肌动蛋白（actin）作为内对照检测DNA质量。

Thereafter an optimized semi-quantitative RT-PCR method was construct; The expressed house-keeping gene β-actin used as internal control, to detect the effects of different zinc source on PR-39 gene expression in weanling pigs.

以此构建一优化的半定量RT-PCR法，以β-actin为内标，研究锌对不同阶段断奶仔猪抗菌肽PR-39基因表达的影响。