hkc的用法

Objective To establish MCP-1 siRNA human renal tubular epithelial cells line and to investigate the inhibitory effect of plasma-mediated small interfering RNA ( siRNA) on the expression of monocyte chemoattractant protein-1 ( MCP-1) gene in human renal tubular epithelial cells ( HKC).

目的建立单核细胞化学吸引蛋白质1（MCP-1）小分子干扰RNA（siRNA）人肾小管上皮细胞株，并检测MCP-1siRNA对人肾小管上皮细胞（HKC）MCP-1基因的抑制效应。

Objective To test the possible role of monocyte chemotactic protein-1 ( MCP-1) and its synergistic effect with aristolochic acid I ( AA ⅰ) on tubular epithelial-myofibroblast transdifferentiation ( TEMT) of human renal tubular epithelial cells ( HKC) in vitro.

目的探讨单个核细胞趋化蛋白1（MCP1）和马兜铃酸Ⅰ（Aristolochic AcidⅠ，AAⅠ）及其两者的协同作用，对人类肾小管上皮细胞（HKC）转分化的影响。

Methods By using flow cytometer ( FCM) bound with Fluo-3 staining, the effects of histamine and the histamine H_2 receptor antagonist cimetidine on the free intracellular calcium concentration of HKC cultured in vitro, were examined half-quantified.

方法利用流式细胞仪（FCM）结合Fluo3染色技术，半定量检测组胺及H2组胺受体拮抗剂西咪替丁对体外培养HKC胞内Ca2+浓度的影响。