egf怎么记

Methods NSCs harvested from the cerebral cortex of neonatal one-week mice were triturated and cultivated in serum-free DMEM/ F12+ bFGF+ EGF+ B27, then identified by the immunohistochemistry SABC method.

方法取新生1周内乳鼠的大脑皮质，机械分离出神经干细胞，无血清培养液（DMEM/F12+bFGF+EGF+B27）培养，免疫组化SABC法对神经干细胞进行鉴定；

The cells in the control group were added with DMEM/ F12 nutrient fluid of EGF, bFGF and B27 to culture in 5% CO2 incubator for one week at 37 ℃.

对照组细胞中直接加含表皮生长因子、碱性成纤维细胞生长因子、B27的DMEM/F12培养液，37℃，体积分数为0.05的CO2培养箱培养1周。

Sample test construction of mutant t-PA cDNA with both F and EGF domains deleted and its expression culture region

t－PA指形区、上皮生长因子样区缺失突变体cDNA克隆的构建及其在哺乳动物细胞中的表达